Ultrasmall gold nanoparticles for highly specific isolation/enrichment of N-linked glycosylated peptides.

Highly specific enrichment of phosphopeptides by zirconium dioxide nanoparticles for phosphoproteome analysis.

Large-scale characterization of phosphoproteins requires highly specific methods for the purification of phosphopeptides because of the low abundance of phosphoproteins and substoichiometry of phosphorylation. A phosphopeptide enrichment method using ZrO2 nanoparticles is presented. The high specificity of this approach was demonstrated by the isolation of phosphopeptides from the digests of mo...

Tools for glycoproteomic analysis: size exclusion chromatography facilitates identification of tryptic glycopeptides with N-linked glycosylation sites.

Proteomic techniques, such as HPLC coupled to tandem mass spectrometry (LC-MS/MS), have proved useful for the identification of specific glycosylation sites on glycoproteins (glycoproteomics). Glycosylation sites on glycopeptides produced by trypsinization of complex glycoprotein mixtures, however, are particularly difficult to identify both because a repertoire of glycans may be expressed at a...

Ultrasmall Gold Nanoparticles as Carriers for Nucleus-Based Gene Therapy Due to Size-Dependent Nuclear Entry

The aim of this study was to determine the size-dependent penetration ability of gold nanoparticles and the potential application of ultrasmall gold nanoparticles for intranucleus delivery and therapy. We synthesized gold nanoparticles with diameters of 2, 6, 10, and 16 nm and compared their intracellular distribution in MCF-7 breast cancer cells. Nanoparticles smaller than 10 nm (2 and 6 nm) c...

Folic acid-modified diatrizoic acid-linked dendrimer-entrapped gold nanoparticles as nanoprobes for targeted CT imaging of human cervical cancer

Highly Sensitive Amperometric Sensor Based on Gold Nanoparticles Polyaniline Electrochemically Reduced Graphene Oxide Nanocomposite for Detection of Nitric Oxide

A sensitive electrochemical sensor was fabricated for selective detection of nitric oxide (NO) based on electrochemically reduced graphene (ErGO)-polyaniline (PANI)-gold nanoparticles (AuNPs) nanocomposite. It was coated on a gold (Au) electrode through stepwise electrodeposition to form AuNPs-PANI-ErGO/Au electrode. The AuNPs-PANI-rGO nanocomposite was characterized by Field Emission Scanning ...